James Ramsbotham, Chief Executive of the North East Chamber of Commerce, says companies like Nissan need to know whether the UK will remain in the EU.
The head of the Japanese car company, Carlos Ghosn, has claimed they would "reconsider" their position if the UK left the EU.
This would threaten jobs at Nissan's plant in Sunderland, which employs 6,500 people.
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The team have responded to three incidents in the last fortnight alone.
The officer was blasted twice by gunman Raoul Moat who had declared war on police minutes before.
Labour's Anna Turley said ministers should have stepped in to stop the "hard closure" from going ahead.