James Ramsbotham, Chief Executive of the North East Chamber of Commerce, says companies like Nissan need to know whether the UK will remain in the EU.
The head of the Japanese car company, Carlos Ghosn, has claimed they would "reconsider" their position if the UK left the EU.
This would threaten jobs at Nissan's plant in Sunderland, which employs 6,500 people.
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Barn Owls are becoming a rare sight, with only 4,000 pairs in the UK. To tackle this some farmers are creating breading areas for them.
Seaside attractions across the North East are to benefit from record £10 million in government investment to create jobs in coastal areas.
QI and Jonathan Creek star Alan Davies has confirmed he will be coming to Middlesbrough for his new show Little Victories in October 2015.