James Ramsbotham, Chief Executive of the North East Chamber of Commerce, says companies like Nissan need to know whether the UK will remain in the EU.
The head of the Japanese car company, Carlos Ghosn, has claimed they would "reconsider" their position if the UK left the EU.
This would threaten jobs at Nissan's plant in Sunderland, which employs 6,500 people.
More top news
The Environment Agency has put a flood warning in place for the River Ouse in York.
Residents are being warned to take immediate action as a severe flood warning has been issued for Boroughbridge in North Yorkshire.
Gluten free food will no longer be available on prescription to adults in Scarborough and Ryedale from next month.