The £1 million Gloriana will lead the manpower squadron of the pageant, ahead of the Queen's barge.
It will be powered by 18 oarsmen including Olympians Sir Steve Redgrave and Sir Matthew Pinsent.
The vessel features wood from sweet chestnut trees grown on the Prince of Wales's private estate.
Spirit of Chartwell
Carrying the Queen and other key royals including the Duke and Duchess of Cambridge, the Royal Barge is lavishly decorated.
The Queen and Duke will be seated on the vessel's top deck in ornate chairs under a gold-coloured canopy.
This paddle steamer will be ferrying the Duchess of Cambridge's family - her parents Michael and Carole and siblings Pippa and James.
The Duke of York, Princess Beatrice, Princess Eugenie, the Earl and Countess of Wessex and other royals will be joined by Boris Johnson and former prime minister Sir John Major and his wife.
It transported Sir Winston Churchill's body along the Thames on his state funeral in 1965.
St Michael's Mount Barge
Built in 1740, the St Michael's Mount is one of the oldest boats on the river and the oldest boat still in commission in Britain. It once carried Queen Victoria - the only other British monarch to reach a diamond jubilee.
Dunkirk "little ships"
Some 40 Dunkirk "little ships" - still waterborne 72 years on - are taking to the Thames, reuniting vessels that evacuated Allied troops from French beaches in 1940.
The Amazon took part in the Royal Fleet Review at Spithead for Queen Victoria's Diamond Jubilee in 1897 - the only Pageant vessel thought to have done so.
Picnickers are enjoying a Heston Blumenthal feast in the Buckingham Palace Gardens before the Diamond Jubilee concert.
The Queen watched a star-studded Diamond Jubilee Concert outside Buckingham Palace on Sunday evening.
Here are some of the best pictures from the Diamond Jubilee River Pageant.