Saimo Chahal, a partner with Mr Nicklinson's solicitors Bindmans LLP, said the next stage of the case would be to prepare an application to appeal against the decision.
She said she would be asking for the case to be expedited so that it can be heard by the Court of Appeal as soon as possible.
Ms Chahal added that Mr Nicklinson's health had deteriorated recently and he was in a great deal of physical pain and discomfort.
She confirmed that he was not prepared to give up his fight to establish the principle of being able to die with dignity when he chooses.
The Courts have left it up to Parliament -so what next for the right to die issue?
Tony Nicklinson, who was left paralysed by a catastrophic stroke, has lost his High Court battle to have a doctor end his life.