The Scotch Whisky Association (SWA) said it was "unfair and incomprehensible" that the Chancellor increased duty on spirits while cutting beer duty.
The SWA claims the Budget announcement penalises consumers who choose Scotch Whisky over beer.
Gavin Hewitt, SWA chief executive, said, "There is no justification for spirits being taxed more heavily than beer".
"It also damages all the good work done to create fairer tax regimes overseas to provide a fairer playing field for Scotch Whisky. It hinders the Government's ambitions for an export-led recovery", Mr Hewitt added.
George Osborne faces criticism that his mortgage guarantee plan might actually make houses less affordable.
The Treasury has still not explicitly said it will prevent homeowners from using taxpayer-backed Mortgage Guarantees to buy second homes.
It opens up the possibility of buying a house to millions who don't have a deposit but they might have to pay more for the privilege.