Andy Burnham has asked Health Secretary, Jeremy Hunt, to substantiate his claims that the Labour MP hid hospital failings in a "cover up".
He said he was concerned that disclosure rules had been broken and challenged Mr Hunt to apologise.
It is impossible to see how you can claim this mounted to a cover-up," he told him in a letter.
I therefore ask you, by the end of today, to provide me with evidence to substantiate your assertion.
If you fail to provide such evidence, I will require a full retraction and public apology. If that is not forthcoming I will consider further action.
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The man, in his thirties, was found with stab injuries on the upper deck of a bus in Handsworth, Birmingham, on Monday night.
Thousands of passengers now face delays, with Southeastern trains warning "all services" would be affected.
Paramedics attended a scene in Willesden, north-west London, on Monday afternoon where they found the teenager with stab wounds.