Police Scotland said earlier restrictions on the flight of EC135 helicopters had been lifted and their helicopter had been cleared to fly.
A spokeswoman said: "As of 3.18pm today ... the Police Scotland Air Support Unit is now fully operational."
More top news
It comes after security was stepped up around the country following the Manchester Arena attack which killed 22 people.
A murderer nicknamed 'Houdini' after escaping the death penalty seven times has been given the lethal injection.
The distress signal sparked a police hunt in the area to find the person or group who made it.