The US Secretary of State has called events in Ukraine a "wake up call" to NATO allies.
John Kerry said allies must make it clear to Russia that NATO territory is inviolable, saying "we will defend every piece".
He said the Ukraine crisis puts "our entire model of global leadership" at risk.
European Union foreign ministers added 13 people to their visa ban and asset freeze list over Ukraine's crisis.
Well that’s the voting over, and no surprises so far. A ‘Yes’ vote claimed to be 89% in Donetsk, and suddenly Ukraine is no more.
Nearly 90 percent of voters in eastern Ukrainian region voted for self-rule, the head of the separatist election commission said.