A statement on the Manchester United website said:
The thoughts of everyone at Manchester United are with the Glazer family tonight following the news that Malcolm Glazer has passed away. He was 85.
The news was announced earlier on the official website of the Tampa Bay Buccaneers, the NFL franchise he acquired ownership of in 1995.
Malcolm leaves behind his wife Linda, six children and 14 grandchildren.
Staff at Manchester United extend deep and sincere condolences to them all at this difficult time.
More top news
Shira Banki, 16, died on Sunday of stab wounds sustained when an ultra-Orthodox Jew attacked a Gay Pride parade in Jerusalem.
HSBC has reported a 10% rise in profits for the first half of the year - boosted by bumper profits in Hong Kong.
Barnado's has received £3 million to tackle child sex abuse in Rotherham, nearly a year after the Jay Report was released.