A statement on the Manchester United website said:
The thoughts of everyone at Manchester United are with the Glazer family tonight following the news that Malcolm Glazer has passed away. He was 85.
The news was announced earlier on the official website of the Tampa Bay Buccaneers, the NFL franchise he acquired ownership of in 1995.
Malcolm leaves behind his wife Linda, six children and 14 grandchildren.
Staff at Manchester United extend deep and sincere condolences to them all at this difficult time.
More top news
David Cameron is to put working people "front and centre" of his economic plan our economic plan" if the Conservatives win the election.
Nearly 38,000 runners will hope to complete Sunday's London Marathon making it the biggest event in its 35-year history.
Rain across central Britain with longer, intense downpours with thunder in places this evening. A lucky few will stay sunny and dry.