Yes campaign leader Alex Salmond has said the independence vote offered Scots "the opportunity of a lifetime".
Beginning his opening address, he said the debate was not if Scotland "could be" but whether it "should be" an independent country.
He said it was wrong that in a "prosperous country of Scotland" young children were still "reliant on food banks" and warned against the UK Government's plan to reinvest billions in funding "weapons of mass destruction" held in Scotland.
Scotland has been "governed by parties that we didn't elect" who brought in policies from "the bedroom tax to the poll tax".
"No one, absolutely no one will do a better job of running Scotland than the people who live and work in Scotland."
The first debate between Alistair Darling and Alex Salmond had barely finished when their respective teams went into battle.
Campaign rivals Alex Salmond and Alistair Darling both traded blows in a surprisingly feisty televised debate on Scottish independence.
Alistair Darling is getting 'feisty' during the STV Debate.