Nicola Sturgeon has accused Theresa May of ignoring Scotland's "voice and interests" as Britain prepares to leave the European Union.
The prime minister told the Conservative party conference in Birmingham there is "no opt out from Brexit" and she will "never allow divisive nationalists to undermine the precious union between the four nations of our United Kingdom".
"We will negotiate as one United Kingdom and we will leave the European Union as one United Kingdom," said Mrs May.
But the First Minister of Scotland hit out at Mrs May's remarks, after the majority of people in Scotland voted to remain in the EU, unlike the rest of the UK.
PM going out of her way to say Scotland's voice and interests don't matter. Strange approach from someone who wants to keep UK together.
Ms Sturgeon said on twitter the prime minister was "going out of her way" to say that "Scotland's voice and interest don't matter".
She added that Mrs May's "approach" was "strange" for "someone who wants to keep the UK together".
The reaction within the Conservative Party to her latest announcements illustrates the problem Theresa May has.
Theresa May has confirmed Article 50, which triggers the withdrawal process for Britain leaving the EU, will be invoked by the end of March.
In other words, the Prime Minister will say that leaving the EU would not lead to a single EU law being abolished instantly.