Raheem Sterling, Fabian Delph and Jordan Henderson have become the latest withdrawals from England's injury-hit squad.
Gareth Southgate's men kick-off their World Cup preparations with back-to-back Wembley friendlies against Germany and Brazil this coming week.
Tottenham's Dele Alli, Harry Kane and Harry Winks have already withdrawn from the initial 25-man squad, with England receiving three further setbacks on Tuesday.
"Manchester City duo Raheem Sterling and Fabian Delph have returned to their club after being ruled out of England's forthcoming fixtures," a statement from the Football Association read.
"Liverpool's Jordan Henderson will also miss out on the back-to-back games against Germany and Brazil at Wembley Stadium through injury."