Darren Lehmann will leave his post as Australia's head coach after the fourth Test against South Africa, which begins on Friday.
Cricket Australia confirmed his departure, which had appeared unlikely when it was announced earlier in the week that he would remain in his post until the end of his contract.
That was due to run until the end of the 2019 Ashes series in England, but now Lehmann has joined the casualties of Australia's Cape Town debacle.
Cricket Australia announced its chief executive James Sutherland would speak to media at 2.45pm BST in Johannesburg following Lehmann's resignation.
Lehmann had been cleared by a Cricket Australia investigation into the ball-tampering, with Sutherland saying on Tuesday the coach had no prior knowledge of the plot.
At the time, Sutherland said it was "inaccurate" to say Lehmann had resigned, but now he has decided to quit.
"It's the right time to step away," Lehmann said, speaking at a press conference. "I'm ultimately responsible for the culture of the team and I've been thinking about my position for a while.
"Despite telling media yesterday that I'm not resigning, after reviewing Steve and Cameron's hurting it's only fair that I make this decision.