A late Kenneth Zohore strike wasn't enough to salvage a draw for Cardiff as Russell Slade's side suffered away defeat at the hands of Brentford.
The game looked destined to end 0-0 before two quick goals from Brentford's Scott Hogan in the last 10 minutes ensured that The Bees took all three points.
The defeat is a blow to the Bluebirds' promotion ambitions, who stay 7th in the Championship table and 6 points off the play-offs places.
Elsewhere in the Football League Newport County ended a run of six successive defeats with a creditable 1-1 draw against promotion chasing Oxford.
Jordan Bowery opened the scoring for Oxford after 13 minutes, but Medy Elito's long-range effort just a minute later made sure the points were shared.
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Jazz Carlin won the women's 800m freestyle gold at the British Swimming Championships in Glasgow but failed to dip below the qualifying time required for the Rio Olympic Games.
The Welsh swimmer touched the wall at 08:27.49 but the Olympic benchmark was set at 08:22.93.
Carlin, who won 200m freestyle gold on Monday and will race again in the 400m later in the week.
It is difficult. I want to be at my best and get on that Olympic team.
It has been a bit of a tussle for a couple of weeks. I have really put a lot of pressure on myself to not miss out on the Olympics, four years ago it was heart-breaking for me.
It has been tough and I just didn't feel myself, when you are racing tense you start to get a bit heavy.
Chloe Tutton set a new British record as she swept to the women's 200m breaststroke title at the British Swimming Championships in Glasgow on Wednesday.
The Cardiff swimmer touched home in 2:22.34mins, taking three seconds off her own personal best, ahead of previous record holder Molly Renshaw.
It couldn't have gone any better at all. I didn't expect this. I trained really hard and I knew I had the potential to do it but I guess I lacked the self-confidence.
Commonwealth and European medallist Georgia Davies won the first British crown of day two in Glasgow as she led throughout the Women's 100m Backstroke final for victory.
I'm so pleased to be back where I wanted to be but there's still a lot more that I need to work on.
There are some girls going really fast all over the world right now at their trials or their nationals, so that's where I want to be.
Although neither Tutton nor Davies achieved the consideration time for Olympic selection, their performances suggested they may yet have a shot of getting on the plane to Rio.
The Football Association of Wales is investigating an alleged breach of its Betting and Integrity rules.
The governing body announced on Wednesday night it was looking into an alleged breach "concerning Port Talbot Town".
"The FAW is liaising with the relevant authorities with regards to this matter and Port Talbot Town are co-operating with the investigation," a statement said.
The FAW added that contrary to some reports, it was not investigating suspicious betting patterns concerning a Welsh Premier League match, saying: "At this stage, the investigation concerns a breach of the FAW's Betting and Integrity Rules."
Port Talbot are expected to respond later on Wednesday.
The Football Association of Wales is investigating suspicious betting patterns on a Welsh Premier League match which took place last Saturday.
Already-relegated Rhyl beat Port Talbot 5-0 at home, having not won in their previous 17 league matches.
We are aware of the issue and are working on this with the relevant authorities.