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Wales captain Alun Wyn Jones faces up to six weeks out with a shoulder injury picked up in the RBS 6 Nations defeat by France in Paris.
The 31-year-old lock was replaced on 51 minutes after hurting himself at a ruck during the 20-18 defeat on Saturday.
Jones, who had been touted as a possible candidate to lead the British and Irish Lions tour of New Zealand, will now not be available for his club Ospreys who are away at Treviso in the Guinness PRO12 this weekend.
Ospreys are set to play the final match of the regular season against Scarlets on May 6, with the Lions squad due to head off to New Zealand around three weeks later.
Six Nations bosses have opened an investigation into the chaotic scenes at the end of the France-Wales game in Paris on Saturday.
Wales' interim head coach Rob Howley said the "integrity of the game had been brought into disrepute" after France replacement prop Uini Atonio went off for a head injury assessment, with starting tighthead Rabah Slimani then returning during a remarkable 20 minutes of second-half stoppage time.
Slimani had earlier been replaced but the France team doctor insisted that Atonio needed an HIA, therefore allowing Slimani to go back on as France laid siege to Wales' line through a series of scrums.
Wales have the backing of royalty as they prepare to face France on the final day of the Six Nations.
The Duke of Cambridge has told French President François Hollande that he will be "cheering the Welsh" tomorrow.
While all hope of Six Nations title success has gone, the runners-up spot behind England - plus a possible top-four world ranking - feature high on Wales' agenda this weekend.
Latest ITV News reports
France's controversial replacement of Uini Atonio by Rabah Slimani in their 6 Nations win over Wales has been referred to a review group.
There will be no further action taken over an alleged bite on George North during Saturday's France v Wales Six Nations clash.