Arriva Buses Wales has announced that 33 staff have been made redundant following the closure of their Aberystwyth depot and outstations at New Quay, Lampeter and Dolgellau.
A spokesperson for Arriva Buses Wales, said "Nine staff will remain to operate the 585 service, which will run until 15 February 2014.
"Four employees have left or transferred to other parts of the business."
The Aberystwyth depot for Arriva Buses Wales will close today, alongside those in New Quay, Lampeter and Dolgellau.
The company says the period of instability regarding public transport funding - together with spiralling fuel costs, has put unsustainable financial pressure on their Aberystwyth operations.