Celtic's Adam Matthews has become the latest player to withdraw from Chris Coleman's Wales squad to face Andorra in the opening Euro 2016 qualifier next Tuesday.
Injury to the defender means a call up for Cardiff City's Declan John from the Under 21's.
Matthews joins Reading forward Hal Robson-Kanu and West Ham defender James Collins who have also withdrawn through injury.
Meanwhile a decision on whether the qualifier will go ahead at Andorra's new national stadium is expected to be made by UEFA today.
UEFA officials have been carrying out pitch inspections over the weekend and have called for more tests of the surface before a final decision is made.
Readings Hal Robson-Kanu has withdrawn from Wales' squad to face Andorra in the opening Euro qualifier next week.
Robson-Kanu hasn't played since injuring his knee during a pre-season friendly with Stevenage.
22 year-old uncapped Reading team mate Jake Taylor is called up in place of Robson-Kanu who joins West Ham defender James Collins on the sidelines.
Chris Coleman's team are in action tonight in a friendly against Iceland at the Cardiff City Stadium.
It's the first time the two sides have met since 2008, when Wales won 1-0 in Reykjavik.
Tonight's game is the start of the build-up for both teams' Euro 2016 qualifying campaign.
Wales manager Chris Coleman has called on fans to support the team in their Euro 2016 qualifying campaign.
Earlier today, Wales were drawn in Group B with Bosnia, Andorra, Cyprus, Israel and Belgium for the qualifying stages. The Euro 2016 finals will take place in France.
"They'll be tough games," he told Sky Sports News.
"Our biggest concerns will be ourselves and how many times we can field our strongest team and the more times we can do that the better chance we can have of getting the points against anybody.
"Bosnia are strong, we've played them already, and Belgium, two strong teams. And looking at Israel they got a couple of good results against Portugal last time in the campaign.
"There's no easy games, but we've got to say we've got a chance if we finish above Israel then we've got a great chance of maybe getting that third spot but that's not to say we're going into it thinking we've got to target third. We've got to win as many games as we can because you never know.
"We took an incredible following to Belgium, there was nothing on the game really, we couldn't qualify (for the 2014 World Cup) and our supporters were brilliant so they'll enjoy some of those trips no doubt."
And Coleman believes the new format for qualification this time could help his Wales side.
"With this new format there's 24 teams so more teams can qualify...and it gives us more of a chance of maybe qualifying and reaching a major tournament," he added.
"Obviously the format that's been before was very difficult for us, but with the extra teams, who knows? It's all up for grabs."
Wales have have found out their opponents for Euro 2016 qualifying, after the draw took place in Nice.
Joining Wales in Group B will be Bosnia, Andorra, Cyprus, Israel and Belgium.