Wales will name their squad later for this Saturday's match against Australia.
The team had to delay the announcement due to several ongoing injury concerns.
The Welsh Rugby Union confirmed that Ospreys prop Aaron Jarvis was ruled out after damaging his knee ligaments during the loss to New Zealand last weekend.
It is yet to be decided if Blues lock Bradley Davies will also be out of contention.
He spent time in hospital on Saturday night and is recovering from severe concussion.
The delayed announcement means that a further six players, who are on the injury list at the moment, could be in a better position to be selected as they've had longer to recover.
Blues centre Jamie Roberts has not been training this week as he remains stiff and sore after leaving the field with a hip injury early on in the All Blacks game.
Ospreys back row Ryan Jones has had stitches after suffering a head wound and will also take a limited part in training until later this week.
Three other players are managing injuries picked up earlier in Wales' Autumn campaign.
Ospreys fly half Dan Biggar has been recovering from a shoulder injury and is hoping to be in contention this later in the week. Fellow Osprey Ian Evans has had a fitness test to see if he can resume training, and Scarlets wing George North is continuing rehab on his hip.
More top news
The world record attempt will raise money for the foundation set up in memory of the Olympic gold medallist, who died in May 2013.
In today's Newsweek, Carl talks about what the 'No' vote in the Scottish Referendum could mean for Wales and the impact of legal highs.
England manager talent spotting at Swansea City v Southampton at the Liberty Stadium