Gethin Jenkins will miss Wales' RBS 6 Nations clash against Scotland at Murrayfield on Saturday.
The Toulon prop, who has won 97 caps, suffered a calf muscle injury during the 26-9 victory over Italy in Rome 10 days ago and is replaced by Bath forward Paul James.
Two other changes from that game see flanker Sam Warburton - last season's Grand Slam captain - return to the starting line-up instead of Justin Tipuric, while lock Alun-Wyn Jones replaces Andrew Coombs.
But even though Warburton has been recalled - he suffered a shoulder injury earlier in the championship - his back-row colleague Ryan Jones remains as skipper.
Wales' interim head coach Rob Howley retains the same back division for a fourth successive game, but three changes on the bench from Rome see Cardiff Blues prop Scott Andrews come in for James, with Coombs and Tipuric swapping places with Jones and Warburton respectively.
More top news
Wales reached their first ever semi-final of a major tournament with a magnificent 3-1 victory over Belgium in Lille.
There'll be clear spells overnight which means temperatures will fall. Setting us up for a dry start to the weekend, but it won't last!
Watch the men who have been making history by taking Wales to the quarter-finals of the European Championships.