London Welsh say they are "extremely disappointed by the Independent Appeal Panel’s decision" to uphold the five-point deduction and £15,000 fine against the club, for fielded an ineligible player, Tyson Keats, in ten Aviva Premiership matches this season.
The decision means that the club remain five points adrift at the bottom of the Aviva Premiership.
In a statement London Welsh said that the RFU's decision to remove the additional suspended five-point penalty imposed until the end of the 2013-14 season, justified their decision to appeal.
The club is hugely disappointed by the decision of the Independent Appeal Panel to uphold the five-point deduction for this season.
We are particularly disappointed for the players, who’ve given everything they can for the club, and ultimately it is they who have been punished for something completely beyond their control.
However, there are still four games remaining in the season and the focus and efforts of the players, the coaching staff and everyone at London Welsh is now on those remaining matches.
More top news
Sunny spells - yes! Fine temperatures - absolutely! Weather warning for rain - of course! There's something for everyone today!
The three are accused of murdering a grandfather in a pub brawl. Wayne Letherby, 42, died after being kicked in the head multiple times.
Striker Gareth Bale has been crowned 'Welsh Player of the Year' for a record fifth time at the Football Association of Wales awards.