Mold Crown Court has heard blood matching April Jones' was discovered in Mark Bridger's living room, with a 'one in a billion' probability match.
Elwen Evans QC, for the prosecution, said Mr Bridger's vehicle was also examined by experts to see if there was any evidence of a collision.
The court heard there was nothing on the bodywork, underside or tyres to suggest contact with a body or a bicycle.
There was also, the court heard, no evidence found in the vehicle, nor any evidence of a large-scale cleanup in there.
The prosecution said there is no evidence to prove April came to any harm at the Bryn-y-Gog estate where she lived, or in Mr Bridger's vehicle.
Earlier today the court heard an account of Mr Bridger's interview with police, in which he said his vehicle had been involved in a collision with April.
The parents of murdered April Jones fear that killer Mark Bridger may never reveal where the body of their daughter is.
ITV News correspondent Carole Green has covered the month-long trial of Mark Bridger at Mold Crown Court. Here she gives her personal view.
The disappearance of April Jones prompted the biggest search of its kind in British policing history.