Wolves defender Sam Ricketts has become the fourth player to withdraw from the Wales squad for next Tuesday's opening Euro 2016 qualifier against Andorra.
The Football Association of Wales says Ricketts picked up an injury in training on Tuesday.
He will be replaced by 35-year-old Danny Gabbidon, who rejoined Cardiff City earlier this week in a player-coach capacity.
Defenders Adam Matthews and James Collins had previously pulled out, together with Reading striker Hal Robson-Kanu. They were replaced by Declan John, Paul Dummett and Jake Taylor, respectively.
Liverpool midfielder Joe Allen, meanwhile, says Wales are confident they can "make big things happen" in their qualifying group.
Gareth Bale believes Wales showed true character to pass their Andorra test and start the Euro 2016 qualifying campaign with victory.
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Wales captain Ashley Williams believes the time is right for Chris Coleman's players to succeed where others have failed.