An animal welfare charity - founded by Queen guitarist Brian May - has threatened legal action if the badger cull goes ahead for a third year.
Lawyers from the 'Save Me Trust' have written to Natural England warning that if they continue with the scheme in Somerset, or activate any new licences, they will be taken to the High Court.
Dorset Wildlife Trust have expressed their disappointed about applications submitted to Natural England to cull badgers later this year.
They work to actively support and promote alternative solutions to badger culling in order to control the spread of Bovine Tuberculosis.
Now in its third year, the wildlife conservation charity has been carrying out a badger vaccination programme in West and North Dorset.
Dorset Wildlife Trust is extremely sympathetic to the farmers whose cattle are affected by this devastating disease, but we urge the Government to consider the scientific evidence which indicates that the cull will not reduce Bovine Tuberculosis in cattle.
The problem could even be made worse as increasing the movement of potentially infected badgers into an area cleared of badgers could risk contact between them and uninfected cattle.
An application to cull badgers in Dorset has been submitted to Natural England.
A number of farmers hope to obtain a licence to kill the animals, which are thought to infect cattle with Bovine TB.
Similar culls in Somerset and Gloucestershire proved controversial, and failed to meet their targets.
Earlier this week, volunteers in the county were given a grant by a wildlife charity to continue their programme of vaccinating badgers against the disease.
Caffe Nero has defended its boycott of milk from badger cull areas as necessary to protect its staff.
In a statement the coffee chain insisted it was not bowing to intimidation, but that it had to act when staff well-being was threatened.
With just 2% of our annual milk supply impacted, we made what we feel was the right choice ... We are not intimidated by protestors in spite of their ongoing and upsetting efforts to threaten our business. At the end of the day, we know that the authorities will support us if needed. However, we made a decision to limit any risk to our people as quickly as possible.
Animal rights activists told Caffe Nero they would protest if the coffee chain continued to use milk from badger cull areas.
Dairy farmers in Gloucestershire and Somerset have criticised activists for their tactics, and talked of boycotting the coffee chain for its decision. Caffe Nero says it has discussed its reasons with the National Farmers' Union.
A West Country MP is criticising Caffe Nero's decision to stop using milk that has come from farms in badger cull zones in Gloucestershire and Somerset.
With two big milk producers in his constituency Ian Liddell-Grainger says the coffee shop's decision will put jobs at risk.
Caffe Nero took the move after anti-cull protesters threatened to boycott the cafe unless the company refused to the use the milk.
I have a lot of farmers in my constituency. I have superb dairymen, superb cattle, wonderful milk. Don't boycott British milk to put British jobs at risk because you've got some petty vendetta. Get a job, grow up and stop annoying the police."
The government has accused animal rights activists of "intimidating and threatening" Caffe Nero into boycotting milk from badger cull areas.
The coffee chain had said it had stopped stocking milk from those farms, after activists said they would protest - leading to anger from farmers in Gloucestershire and Somerset.
It is wholly unacceptable for a small group of protestors to intimidate and threaten retailers in this way.
Our strategy for tackling bovine TB is based on advice from the Chief Veterinary Officer about the best way to control this harmful disease which threatens the future of our dairy and beef industries.
We will continue to work closely with the dairy industry and retailers to offer them all the support we can.
Caffe Nero says it has stopped stocking milk from farms in badger cull areas, after animal rights activists said they would protest - leading to anger from farmers.
The coffee shop chain now faces a possible boycott from farmers in Gloucestershire and Somerset, including the President of the National Farmers' Union.
The NFU's Deputy President has argued that the cull is essential for eradicating TB in cows, and a lifeline to farmers.
"The people who have made the threat of action against Caffè Nero are a small minority and it is extremely disappointing that the company appears to have bowed into pressure..."
“We need to remember that we are talking about controlling bovine TB – a disease which is spreading in cattle and badgers and will continue to spread if left uncontrolled. The pilot badger culls are a government policy, based on scientific evidence, aimed at controlling this disease which is a huge threat to dairy and beef farmers in the South West and other parts of the country."
“It is especially saddening that this has come at a time when the dairy sector is facing price pressures ... We would urge all members of the supply chain to continue support for British dairy farmers.”
Guitarist Brian May will be in Gloucestershire today to talk to fellow protesters against the badger culls.
It follows the start of his latest campaign, called Common Decency. It aims to encourage more people to vote and urges politicians to abide by certain principles including a commitment to animal welfare.
Campaigners fighting to stop the badgers culls in Somerset say they've had a 'constructive' meeting with the Environment Secretary.
Members of the Badger Trust spoke to Liz Truss in her London office, claiming the culls are both costly and ineffective in halting the spread of TB in cattle.
Afterwards they said she had agreed to consider various points about animal welfare and vaccination.
She said the chief veterinary officer still continues to advise her that it is a tool that needs to be used. She made no commitment on extension of the culls at this stage even though we did agree if there were any steps to go forward they'd have to go through proper licencing and consultation processes. She did accept that cattle measures are important, she did accept badger vaccination has a valuable roll to play as well which is a step forward from her predecessor Owen Patterson.
The Labour Party will today promise to end the controversial badger culls in Somerset and Gloucestershire if it wins the General Election.
It's one of six commitments to protect animals being announced on the tenth anniversary of the ban on fox hunting coming into force.