The Christmas Eve accident on the M5 near Stroud involved six vehicles travelling northbound. At least two vehicles on the southbound carriageway hit debris from the initial accident.
A 38 year-old woman driving a Citroen Berlingo van was seriously hurt. The woman from Ross-on-Wye was taken to Southmead Hospital in Bristol for emergency surgery. She is said to be in a 'very serious and critical' condition.
Other people with minor injuries were treated at the scene. The motorway was finally reopened at 3am on Christmas morning - eight hours after the crash.
Police investigators are appealing for information from anyone at the scene, including drivers who may have dashboard cameras.
"To attend an incident such as this at any time of year is incredibly sad and traumatic. It will undoubtedly have affected many people who travelled on the M5 and particularly those who were involved in it and saw it happen."
A woman has undergone emergency surgery after a Christmas Eve pile-up in Gloucestershire that closed part of the M5 for eight hours.
The crash involving five cars and a van happened on the northbound carriageway between Jct 13 Stroud and Jct 12 Quedgeley at around 7pm.
The woman, who is 38 and from Ross-on-Wye, was taken to Southmead Hospital in Bristol. Two other people were hurt.
Jesse Burrows from Minchinhampton near Stroud has been found safe and well.
The alarm was raised after Jesse, who is pregnant, disappeared yesterday without taking her medication with her.
Gloucestershire Police would like to thank everyone for their concern and help.
The funeral takes place today of a brother and sister killed in a crash near Stroud. Their mother is determined it will be a happy event.Read the full story ›
The funeral of a brother and sister killed in a car accident in Stroud takes place today.
Jinny-Mae Cook, who was 18, and her brother Bobby Roberts who was 28 died when their car collided with another vehicle on the Ebley bypass almost two weeks ago.
Their mother was almost killed 4 years ago when she was involved in a hit and run accident, and dragged 100 feet under the wheels of a car. She says she's is overwhelmed by all the support she's received.
"The love for me, the love for my children, everyone's love has and is bringing me through this. What kind of day do you want this to be? Oh it is going to be a happy event, there's not going to be sadness, it's going to be happy."
The Prince of Wales has been out and about in his home county of Gloucestershire.
He met schoolchildren, business reps and organisers before opening Festomane, The Stroud Festival of Manufacturing and Engineering. It was founded in 2012 to promote Stroud's heritage and showcase local employment and training in the sector.
A teenage girl and her brother have died in a car crash which has devastated their mother. She herself survived being run over by a car four years ago.
18 year old Jinny-Mae Cook and her brother Bobby Roberts died on Saturday when their car collided with an oncoming vehicle near Stroud.
The accident has devastated their mum Julie Bloodworth. She herself survived being run over by a car in 2010. She was found in the Parliament Street car park in Stroud on the brink of death.
On Saturday, she was told that Jinny-Mae and Bobby were pronounced dead at the scene of the crash on the Ebley by-pass.
In February 2010 Julie suffered a broken skull, holes in her left shoulder and back, three broken ribs, ligament damage to her left leg and a torn left ear.
Police believe she was run over near her Brick Row home and dragged along by the vehicle.
The driver was never found.
Police are appealing for information about Saturday's crash on the A419 Ebley bypass between Stonehouse and Stroud at around 6.45pm.
It involved the silver Volkswagen Golf in which Bobby and Jinny-Mae were travelling towards the Dudbridge roundabout and a blue Vauxhall Vectra travelling in the opposite direction towards Stonehouse.
Two people have been killed in a car crash in Stroud.
The collision between a Volkswagen Golf and a Vauxhall Vectra happened on the Ebley bypass between Stonehouse and Stroud at about 18:45 on Saturday.
The man driving the Volkswagen and his female passenger were pronounced dead at the scene.
The male driver and male passenger of the Vauxhall Vectra were taken to hospital with serious injuries.
Gloucestershire Police urged anyone with information to contact them.
A man and a woman have died in a two car crash near Stroud last night.
They were both in a silver VW Golf which was in collision with a blue Vauxhall Vectra at about 6.45 pm on the Ebley by-pass between Stroud and Stonehouse.
A spokesman for Gloucestershire police said: "The Golf was travelling towards the Dudbridge roundabout and the Vectra was travelling in the opposite direction towards Stonehouse.
"The male driver and the female passenger of the Volkswagen sadly died and were pronounced dead at the scene. A male passenger in the Golf was taken to Gloucestershire Royal Hospital with serious, but not life-threatening injuries.
"The driver of the Vauxhall, a man, was also taken to GRH with injuries described as not life-changing.
"Anyone who may have information on this incident is asked to contact Gloucestershire Police on 101"
The names of the two people who died haven't yet been released.
A man fell from a house at Cheapside, in the Hill Paul area of Stroud this morning.
Emergency services were called to the incident at 11.40 am.
The man received serious chest and stomach injures in the fall.
He was treated by paramedics at the scene and airlifted to Southmead Hospital in Bristol.
Police say they're not treating it as suspicious.