Yeovil Town's final game in the Championship ended with a heavy 4-1 defeat to Middlesbrough at Huish Park. James Hayter scored for the Glovers.
In League One, Bristol City drew 1-1 at Crawley. Jamie Proctor gave the home side a first half lead, but Simon Gillett equalised for City just after the hour mark. Steve Cotterill's men finish the season in 12th place.
Former Cheltenham striker Wes Thomas scored both goals for Rotherham as Swindon Town lost 2-1 at the County Ground. Town pulled a goal back through Michael Smith's penalty. Mark Cooper's men finish 8th in the table.
In League Two, Bristol Rovers have been relegated from the Football League after losing at home to Mansfield. With relegation rivals Wycombe and Northampton both winning, it means Rovers drop into the Conference along with Torquay United.
Cheltenham were leading twice against Dagenham and Redbridge after first half goals from Steve Elliott and Matt Richards, but ended up losing 3-2. Town finish the season in 17th place.
Shane Monahan scored a hat-trick of tries as Gloucester beat London Irish 38-30 at Kingsholm.
Bath were unable to confirm their place in the Aviva Premiership play-offs after drawing with Northampton at The Rec on Friday night.
Bristol Rovers' battle to stay in the Football League has received a huge boost after they beat relegation rivals Wycombe.