Two trials of badger culling can legally get underway on Saturday 1 June.
Natural England has given the culls final approval, and chosen Dorset as a reserve culling area.
A Government minister has hinted that the region's trial badger culls scheduled for later this year may NOT go ahead in the planned areas.
Queen guitarist Brian May could be heading for an unlikely hit after his protest song inspired by the badger cull reached the charts.
The song, Save The Badger, Badger, Badger, was recorded by May and internet entertainer Weebl and features the vocals of Brian Blessed.
It has made the top 40 in the iTunes download chart and is the most popular track in the iTunes Store top 10 rock chart.
Blessed, who played Prince Vultan in the 1980 film Flash Gordon, said: "Brian May is absolutely inspirational and together we will beat the dark forces and save the badgers."
Around 5,000 of the animals are expected to be killed in controlled shootings over six weeks in Somerset and Gloucestershire.
Supporters say the cull is needed to tackle bovine TB, which can be spread from infected badgers.
May said: "The British people are speaking in their many thousands, and yet the government is refusing to listen.
"We thank them for buying this track and giving the badgers a voice. Let's get this to number one so (David) Cameron cannot avoid it."
The Liberal Democrat MP for West Cornwall, Andrew George, has said the Government's decision to carry out a badger cull to try to eradicate Bovine TB actually risks making things worse.
He also said good progress was being made to vaccinate Badgers against TB in Penwith.
Campaigners camped out on land at Watchet in Somerset have said they will probably relocate on The Crown Estate.
The Crown Estate announced it would allow the cull to take part on its land.
A spokesman for the campaign group said they would not seek permission to use the land from The Crown Estate.
– Jay Tiernan, spokesman for campaigners.
It will be a protest camp and they are often on other people's properties.
The premises we are on at the moment is not involved in the badger cull, but the next person's land will be.
We are not interested in having conflict with people who are not in the badger cull."
Protesters have spent another night patrolling parts of West Somerset in an effort to stop the badger cull.
Sara Palmer from Somerset Badger Patrol said they will do whatever they can to disrupt the work of the marksmen:
A group of protesters campaigning against the badger cull has agreed to leave 'Camp Badger' at Watchet on Wednesday afternoon.
It is after the landowner asked them to leave.
Members of the Green Party in the West Country said they supported alternatives to culling such as controls on movements of cattle and vaccination.
– Molly Scott Cato, Leader of the Green Group on Stroud District Council.
In view of the likely local opposition to the badger cull in my local area I find it deeply regrettable that the National Farmers Union (NFU) decided to try to limit peaceful protest on this issue and welcome the judge's ruling on the injunction that the right to peaceful protect must be protected.
The attempt to suppress peaceful opposition is a sign of weakness and it shows that the NFU knows the public, like the Green Party, is against the cull.”
A protester has told ITV's Daybreak that the badger cull is "unscientific, unethical and illegal" after the six-week pilot scheme began last night.
A number of protesters have vowed to remain in "Camp Badger" in west Somerset for the duration of the cull.
Several animals were believed to have been killed in the area last night, leaving the protester "sickened" and "devastated".
Anti-cull campaigners have been out on patrol overnight in West Somerset, trying to disrupt the badger cull. The Somerset Badger Patrol had at least three groups out, coordinated on social media websites, walking along public footpaths beside farmland.
Marksmen have begun the cull of badgers in two trial zones to try to control the spread of TB in cattle.
Our presenter Ian Axton has been speaking to Andrew Butler from the National Farmers Union. He began by asking him why he thinks the cull will work....