The Cornish Pirates have been beaten by Bedford in tonight's Championship clash.
The hosts scored two late tries, with 22 points coming from the boot of Jake Sharp.
Keiron Hallett kicked two penalties in reply for the Pirates.
The Cornish Pirates won 32-6 at Dundee High, while Plymouth Albion suffered a heavy 59-26 defeat at Rotherham.
It's not been a good day for our Championship teams.
The Cornish Pirates lost 25-24 at London Scottish, with the home side kicking an 80th minute penalty to win the game.
Local Newlyn youngster Jack Nowell had earlier scored a debut try for the Pirates.
Meanwhile, Plymouth Albion suffered a heavy 55-17 defeat at Bedford.
Canadian winger Matt Evans scored three tries as the Cornish Pirates began the Championship season with a comfortable win at newly promoted Jersey.
A strong second half for the home side. Three penalties plus a try from Hudson Tonga'uiha killed the game off.
The Pirates didn't give up and pulled a late try back from Ryan Storer, but this was cancelled out by Jack Moates.
So defeat on the night and on aggregate secures London Welsh the Championship trophy.
Welsh are now expected to take legal action over the RFU's ruling that they are not eligible for promotion to the Premiership.
The Pirates have reduced the deficit to nine points at the break. Aaron Penberthy gave them a fourth minute lead with a drop goal.
Rob Cook kicked a penalty, and it was his storming run in the 23rd minute that set up Matt Evans for the only try of the half, which Cook then converted.
Tonight's match at the Kassam Stadium has kicked off. The Pirates are hoping to overturn a 16 point deficit as they take on London Welsh in the second leg of the Championship play-off final.
In rugby, the Cornish Pirates are hoping to overturn a 16-point deficit when they take on London Welsh in the second leg of the Championship play-off final tonight.
The Pirates lost last week's first leg at the Mennaye Field 37-21.
Tonight's game takes place at the Kassam Stadium in Oxford.