The captain of a migrant boat which capsized off the coast of Libya killing hundreds onboard has been charged with reckless multiple homicide, the Associated Press reports.
Assistant Prosecutor Rocco Liguori said the Tunisian national and a Syrian crew member were also charged with "favoring illegal immigration".
As many as 900 people died in the tragedy on Sunday while just 27 survived.
Italian Prime Minister Matteo Renzi has compared the smuggling of migrants across the Mediterranean to the African slave trade centuries ago.
He said: "When we say we are in the presence of slavery, we are not using the word just for effect."
On Sunday Renzi appealed to other European countries not to leave Italy alone in dealing with the crisis prompting emergency talks on the matter.
Two survivors of the migrant boat disaster which killed around 900 people on Sunday have been arrested on suspicion of people trafficking, Italian authorities say.
Officials from the prosecutor's office said the men were the captain and first mate of the vessel, according to Reuters.
The 27 survivors of a migrant boat which capsized killing as many as 900 people are finally back on dry land tonight 24 hours after the tragedy.
ITV News Correspondent John Ray tweeted it was the "end of a journey from hell".
24 hrs after their boat capsized killing 900 the few survivors finally reach Italy. End of a journey through hell http://t.co/Ap83Canhy1
David Cameron has been urged to "prioritise saving lives" by a coalition of aid agencies and refugee groups when he attends emergency talks on the migrant boat crisis.
An open letter to the Prime Minister, co-ordinated by the British Refugee Council, said a way must be established to help refugees fleeing turmoil to find protection in Europe safely and legally.
The groups, including Oxfam and Amnesty International UK, say there needs to be a new approach to dealing with the issue.
"There is no easy answer to this crisis, but the solution can never be to impassively watch on while desperate people drown," the letter says.
"Until safer ways to seek refuge are found, more people will be forced into the hands of smugglers and onto unseaworthy boats in their search for a safe haven.
"At the moment, European and Britain's immigration policies leave many with no other choice.
"Building the walls of fortress Europe has had disgracefully deadly results. As we've seen, it's a matter of life and death."
America has pledged an additional $17.7 million to provide the Ukrainian government with aid for essentials such as food, shelter and water.
Vice President Joe Biden spoke with Ukrainian President Petro Poroshenko about reform efforts in the country today.
A statement from the White House said: "The vice president welcomed the appointment of a new head of the anti-corruption bureau and encouraged the further implementation of rule of law reforms, including anti-trust measures and judicial reform."
The Royal Navy should be sent to the Mediterranean to help thousands of migrants following the latest boat disaster, Nigel Farage has said.
After accusing David Cameron yesterday of "directly causing" the deaths of migrants in the Mediterranean Sea by bombing Libya in 2011, the Ukip leader said if he was Prime Minister he would send the Royal Navy to help.
Speaking in Rochester, Mr Farage said: "We could help. We could send the Royal Navy down, but it's a question of what's the signal - is the signal 'If they keep on coming we'll keep on taking you' or is the signal 'We're very sorry, we can't take you'?"
Asked what he would do as Prime Minister, Mr Farage said: "I would say there is a horrible humanitarian crisis going on, we'll send the Royal Navy - what's left of it - to help, we will divert part of our £20 billion a year aid budget."
He said Mr Cameron has a "very big challenge" when he attends a summit on the migrant crisis at the European Council in Brussels on Thursday.
The migrants come from all over Africa and the Middle East guided by traffickers to the coasts of Tunisia and Libya. They are herded on to over crowded and often unsafe boats and aimed for the Italian islands of Lampedusa or Sicily.
Home Secretary Theresa May has responded to claims that European nations have not done enough to tackle the migrant crisis saying: "Ministers have agreed that there is no quick fix on this issue."
Europe's leaders were urged by the UN to have a 'less callous' approach to the surge of migrants. At a meeting in Luxembourg, EU ministers agreed to hold an emergency summit on Thursday. The European Commission presented ministers with a 10-point plan for immediate action to ease the migrant crisis in the Mediterranean.
ITV News International Affairs Editor Rageh Omaar reports from Luxembourg: