Wycombe should have been deducted points and relegated to the Conference instead of Bristol Rovers for breaching the Football Association's rules on third party ownership, the Pirates are set to tell the Football League and Football Association.
In a dramatic final day of the League Two campaign last month Rovers fell out of the Football League for the first time in their history on goal difference, with the Chairboys surviving by the same method.
Four weeks later Wycombe admitted breaching FA regulations during the sale of winger Matt Phillips to Blackpool in the summer of 2010 and decided not to appeal their fine of £10,000 at a disciplinary hearing.
The Blues, who pointed out to the hearing that they are now under new ownership, were also warned about their future conduct and the agent involved has had his licence suspended.
But Bristol Rovers have suggested that the serious breach in the Phillips deal and "possibly other players" gave Wycombe an unfair advantage over them and will make a formal complaint to the authorities in a bid to secure a shock return to League Two in place of Gareth Ainsworth's men.
"It has come to the attention of Bristol Rovers FC that Wycombe Wanderers FC appear to have breached FA rules on Third Party Ownership," their statement read.
"It's current chairman, in giving evidence at a recent FA Disciplinary Hearing, appears to have admitted a serious breach in respect of the player, Matt Phillips (and possibly other players).
"The result of this led to Wycombe wrongfully obtaining a competitive advantage over Bristol Rovers.
"Without this, Wycombe rather than Bristol Rovers could have been relegated.
"Bristol Rovers were relegated merely on goal difference, and as the breach, if proven, would have almost certainly led to a points deduction, Bristol Rovers have instructed specialist sports lawyers together with Nick De Marco, a leading sports barrister, to draft and send a complaint to both The Football League and The Football Association."
There was no immediate comment from Wycombe when contacted.