Stoke City's reward for their FA Cup fourth round victory over Arsenal is an equally tricky tie with Manchester City.
Stoke will travel to Eastlands for the match against City, who are unbeaten in cup ties so far this season.
Meanwhile, cup holders and favourites for this year's tournament, Chelsea, received a relatively kind draw. They will take on 2008 finalists Cardiff at Stamford Bridge.
South Coast football fans will be looking forward to a derby between arch rivals Southampton and Portsmouth, while Aston Villa face a trip to Wolves or Crystal Palace.
The winner of the replay between Leeds United and Tottenham will travel to Bolton, while Notts County, the lowest ranking team left in the competition, will take on Fulham if they manage to get past Wigan in their replay.
Reading's prize for overcoming Premier League sides Liverpool and Burnley is a home tie against fellow Championship side West Brom.
FA Cup Fifth Round Draw (ties to be played on 13th and 14th February 2010)
Wolverhampton Wanderers or Crystal Palace v Aston Villa
Manchester City v Stoke City
Derby County v Birmingham City
Bolton Wanderers v Tottenham Hotspur or Leeds United
Chelsea v Cardiff City
Fulham v Notts County or Wigan
Reading v West Bromwich Albion
Southampton v Portsmouth
