Defender Olly Lancashire and midfielder Peter Sweeney are suspended for Grimsby's FA Cup first-round tie at home to non-league Bath City.
Lancashire, on loan from Southampton, would have been eligible to play had he not been sent off during last weekend's league draw against Accrington.
Sweeney received his fifth yellow card of the season against Stanley and both players must serve one-match bans.
Rob Atkinson's recovery from a groin injury is timely and the 22-year-old comes straight into contention to replace Lancashire.
Barry Conlon staked a claim for a recall by marking his return from a four-match suspension with Town's last-minute equaliser against Accrington after coming on as a substitute.
Grimsby have won their opening game in the competition just twice since 2000.
Bath City's Kaid Mohamed will undergo a fitness test on the morning of the game.
The striker has been out for the last three weeks with a thigh injury but manager Adie Britton is hoping he can come through a fitness test on Saturday morning.
Should Mohamed fail to make it, Mike Perrott or Stuart Douglas could deputise.
Although, having played just 20 minutes since returning from a neck injury, Douglas may not be ready to start the game.
Britton's other selection concern is centre-back Gethin Jones, who has not trained this week due to a virus.
But City boss is hoping Jones will return to training prior to the game. Should he fail to recover in time 38-year-old Marcus Browning will step up from the bench.
Despite the disruptions, Britton insists preparations for the game have been good and and he is eyeing an upset.
"We are running into some form and looking forward to the challenge," he said.
"Obviously it is a major task for us but who knows. If they have a bad day perhaps there is a chance."M
