Tranmere scored a controversial late winner to see off Sky Bet League Two side Accrington in a 1-0 win.
Accrington Stanley live updates
Former Accrington managing director Robert Heys has lost his appeal to reduce the 21-month ban imposed on him by the Football Association for breaking betting regulations.
In August, Heys, who had been involved with Stanley for over a decade, was suspended for all football activity for nearly two years after he admitted to breaching 735 betting rules, including wagers placed on his own club to lose.
Heys resigned from his role at Accrington in the wake of the news and despite attending an appeal hearing to reduce his ban on October 8, his full punishment, which also included a £1,000 fine, was upheld.
Accrington managing director Robert Heys has been suspended from all football activity for 21 months by the Football Association after breaching their betting regulations.
The governing body charged Heys at the start of the month and he admitted to 735 breaches of their betting rules at a hearing last Thursday.
Stanley's managing director, who was also fined £1,000, intends to appeal the punishment.
James Beattie has expressed his delight after claiming his first win as manager of Accrington Stanley.
The League Two side beat Middlesbrough 2-1 at the Riverside in the First Round of the Capital One Cup on Tuesday night.
Accrington Stanley's managing director Robert Heys has been charged with a number of betting offences by the Football Association.
The FA's rules prohibit players or officials betting on any matches in competitions with which their club is involved.
Heys, who has been at the League Two club for more than 10 years, has been charged with alleged breaches of FA rules in relation to a number of betting offences and has until August 9 to respond to the charges.
The new Football League season kicks off this weekend.
Sammy McIlroy has been assessing the chances of the north-west's League Two sides.
Accrington have completed the signing of striker Danny Webber on a one-year deal following a successful trial with the club.
Webber, 31, joins the club as a free agent after leaving Doncaster this summer and has been training with the Reds throughout pre-season.
Accrington have completed the signing of midfielder Josh Windass on a one-year deal.
Windass, 19, has played a key role in Stanley's pre-season schedule and has been rewarded with a contract at the Store First Stadium.