The Odense zoo in central Denmark says the aim is to give people "a closer-to-the-animals experience".
The female lion was put down nine months ago because the zoo had too many felines and failed to find a new home in Europe for the animal. The animal has since been kept in a freezer, and will be dissected on Thursday to coincide with the schools' autumn break.
The event has attracted several protests but has been mostly well received in Denmark, unlike similar plans at the Copenhagen Zoo in February 2014.
Zookeeper Michael Wallberg Soerensen said the Odense Zoo has performed public dissections for 20 years and that they are "not for entertainment" but are educational.
"We are not chopping up animals for fun. We believe in sharing knowledge," Wallberg Soerensen said. "It is important not to give animals human attributes that they do not have."
Many Danes posted positive comments on Odense Zoo's Facebook page, with some agreeing that children will not be harmed watching the dissection.
The zoo undertakes dissections once or twice a year, often attended by Kindergartens and school pupils.