More than four million people who signed a petition pushing for a second referendum on the Brexit have been told that their calls have been rejected in an official response from the government.
Normally parliament would debate any petition on its official site which gained more than 100,000 signatures.
However a formal response from the Foreign and Commonwealth Office made clear that there is no prospect of a re-run on the close vote.
It added: "As the Prime Minister made clear in his statement to the House of Commons on 27 June, the referendum was one of the biggest democratic exercises in British history with over 33 million people having their say."
The petition, set up by William Oliver Healey, states: "We the undersigned call upon HM Government to implement a rule that if the Remain or Leave vote is less than 60% based on a turnout less than 75%, there should be another referendum."
In the EU referendum, 51.9% of votes were cast to leave the EU, versus 48.1% for remaining part of the bloc.