The terraces at Wembley fell silent for one minute in remembrance of the Manchester terror attack victims.
The Duke of Cambridge also laid a wreath to honour the victims before kick-off.
Tens of thousands of supporters descended on the venue to watch Saturday's match between Arsenal and Chelsea, in the first major city event since the terror attack on Manchester arena which left 22 dead and injured dozens more on Monday.
The match was guarded by armed police despite the country's threat level being reduced.
The Duke of Cambridge will be among the 90,000 spectators.
On the ground, officers were seen patrolling the entrance on horseback, amid chanting and a sea of blue and red shirts.
A beefed-up security operation saw a police helicopter circling the skies above the grounds in the hours leading up to kick-off.
Chelsea fan Gerry Morrissey, who travelled from Dublin for the occasion, said he felt "secure".
The 64-year-old said: "I'm delighted to be here. It's nice to see the police around here, but I'm not too worried.
"There's a nice (police) presence here, not overpowering. It feels secure. They're used to big crowds here at Wembley so I feel very confident."
Spectator Mike Purtill, 51, said: "I do feel safe. I don't think you can allow something like that to stop you going on with your day to day.
The Arsenal fan, from London, said he was more concerned about the "Chelsea forward line" than something happening to him.
He said: "I've lived in London long enough to be around in the 1980s and 1990s when there were other bombs going off.
"You can't let it stop you from getting on with your life.
"There's always a high level (of security) at these sorts of games, but there's probably more police with guns than are usually seen.
"I wouldn't say I've seen more police, but I suppose they could be, also, being somewhat discreet."
Security was reviewed at more than 1,300 events and around 1,100 armed officers were freed up to be deployed nationwide.