Culls will take place in areas in Avon, Cheshire, Cornwall, Staffordshire, Devon, Dorset, Herefordshire and Wiltshire, under licences published by government agency Natural England.
The controversial cull of the wild animals will now take place in 40 areas across England this year following the reauthorisation of licences in 29 existing areas.
Expansion of the cull, aimed at preventing transmission of the disease to livestock, was met with anger by wildlife campaigners.
But farming minister George Eustice said: “Bovine TB remains the greatest animal health threat to the UK, costing taxpayers over £100 million every year as well as causing devastation and distress for hard-working farmers and rural communities.
“There is no single measure that will provide an answer to beating this disease.
“That is why we have always been committed to a multi-pronged approach including proactive badger control as well as other tools such as tighter cattle controls, improved biosecurity and badger vaccination.”
An recent independent review, commissioned by the Government of its strategy for tackling TB in livestock amid, warned against an “over-emphasis” on the role of wildlife and found badgers were not the primary cause of problem.
Professor Sir Charles Godfray, who led the review, said spread of the disease between cattle was more of a problem than badgers and farmers must do more to tackle the spread of tuberculosis between livestock.
Experts involved in the review last year suggested the totality of the evidence on badger culling showed a real but modest effect in curbing TB.
Mr Eustice said the Government would be responding to the review in the near future.
Ellie Brodie, senior policy manager at The Wildlife Trusts, said: “Evidence shows that badgers are not the primary cause of the spread of TB in cattle and that the primary route of infection is from cow-to-cow contact – so a vaccine for cattle should be a government priority.”
She said Wildlife Trusts had been and would continue to vaccinate badgers on their reserves and in partnership with vets, farmers and landowners.
In Derbyshire, an application to cull was refused at the last minute last week amid a Government review of the role of culling in an area where a vaccination scheme was already under way.
Badgers likely to be killed in the latest culls
The number of areas licensed to cull badgers
Quizzed by MPs on the move to block the Derbyshire cull, Environment Secretary Theresa Villiers said the Government’s overall policy was “unchanged” and the decision related to local circumstances.
Dominic Dyer, of the Badger Trust, criticised the Government signing off on “the largest destruction of protected species in living memory”.
He said as a result of the new licences more than 60,000 badgers were likely to be killed, bringing the total to some 130,000 since the policy was introduced in 2013.
And he said: “For the Government to carry out such a huge slaughter of a protected species at a time when no parliamentary scrutiny can take place is an unforgivable act of ecological vandalism and a national disgrace.”
Earlier this week, experts warned thousands of badgers were experiencing “extended suffering” in the cull, taking more than five minutes to die after being shot, and questioned how effective the policy was.