The team ran out with the words “Be Kind” printed across the back of their shirts, as part of the club’s campaign to raise mental health awareness in partnership with the Samaritans.
He is one of a wave of famous faces who have urged kindness from the public and media following Flack’s death.
Cipriani said ahead of the match: “Caroline was one of the most caring, genuine people I ever met.
“She endured unimaginable criticism during her years in the public eye but through it all, she just wanted people to show a little kindness to each other. And that’s a message that should be her legacy – be kind.
“We all have spells of self-doubt, anxiety and low points. Nobody needs other people to point out their flaws and make them feel worse about themselves. A little kindness goes a long way.”
The Gloucester Rugby Foundation teamed up with the Samaritans to heighten the awareness around mental health.
They are donating £5 from every ticket purchased to the Gloucester and District Samaritans.
Off the pitch, Gloucester wore blue training tops featuring the Samaritans logo.
They had previously planned to wear black armbands in honour of Flack.
Cipriani recently opened up about his own struggles with mental health in an emotional 18-minute video posted on social media.
Following Flack’s death, he also released a screenshot of a recent WhatsApp exchange between the pair, and said he was “grieving hard”.
Flack stepped down from presenting the winter series of Love Island following an alleged assault on her boyfriend Lewis Burton.