Colchester United have confirmed four people at the club have tested positive for coronavirus.
In a statement released on Saturday night, the U's said 28 players and staff were tested at the club's Florence Park training ground over the course of Thursday and Friday, and four individuals tested positive for the virus.
They will all now self-isolate at home for the next seven days, and will only be allowed to return to the training ground when they've tested negative.
The rest of the Colchester squad will return to training on Monday as planned ahead of their proposed League Two play-off semi-final against Exeter City.
League Two clubs recently indicated they still wanted the play-offs to go ahead, but the decision still needs to be ratified by the EFL.
"Of the 28 Colchester United players and staff that completed tests at Florence Park earlier this week, four returned positive results for the virus," a Colchester United spokesperson said.
"In line with the EFL protocols surrounding a return to training, they will now self isolate at home for the next seven days."
In total, there were seven positive tests across the four teams involved in the League Two play-offs after the first round of testing - including one at Northampton Town.
The Cobblers have revealed physio Ignacio Herrando was the person who tested positive and he will now self-isolate at home for the next week.
According to the club, Mr Herrando is asymptomatic and is feeling well.
Like Colchester, Northampton will return to training on Monday.