Watch a full report from ITV News Anglia's Rob Setchell.
Crew members for the East Anglian Air Ambulance are moving into their newly built headquarters at Norwich Airport.
The seven million pound base will mean the charity can start flying 24-7 from late June.
By operating through the night the charity estimates it could be used up to six hundred times more each year.
The opening is six months behind schedule due to the pandemic.
The EAAA currently flies up until midnight from the Cambridge base, but there is no air cover through the early hours of the morning, with rapid response vehicles used instead.
Planning was officially granted for the base in November 2019 with ground works starting in January 2020.
Construction was halted by the coronavirus pandemic but was only pushed back to six months behind the original schedule.
EAAA aims to raise £15 million a year to deliver and develop its service.
The new purpose-built base brings all of its Norwich-based teams under one roof for the first time.
The base also includes a community hub and training and conference facilities, as well as an immersive training suite for its clinical teams.