The First Minister has confirmed SNP MPs will not back Boris Johnson's new Brexit deal.
Nicola Sturgeon said it was "not acceptable" for Scotland to be facing an outcome it did not vote for.
Following days of negotiation, the Prime Minister and the EU agreed on a deal that will now be presented to European leaders.
Boris Johnson asked Parliament to back his "great new" Brexit deal that he claims "takes back control" - but the agreement is not supported by Northern Ireland's DUP or Labour.
He tweeted: "We’ve got a great new deal that takes back control — now Parliament should get Brexit done on Saturday so we can move on to other priorities like the cost of living, the NHS, violent crime and our environment #GetBrexitDone #TakeBackControl"
In response to the deal, Sturgeon made it clear, again, that her ideal solution is for Scotland to be independent, while she also supports "efforts to ensure peace and stability on the Ireland of Ireland".
The statement read: “While there remains uncertainty over whether this proposed deal will pass what is absolutely clear is that it would take Scotland out of the European Union out of the single market and out of the customs union against the overwhelming democratic will of the people of Scotland.
“Scotland did not vote for Brexit in any form, and SNP MPs will not vote for Brexit in any form – especially when it is clear that Scotland, alone of the nations of the UK, is being treated unfairly.
“We support efforts to ensure peace and stability on the island of Ireland, in line with the Good Friday Agreement, which must be respected.
“At the same time, it cannot be right that Scotland alone is facing an outcome it did not vote for – that is democratically unacceptable and makes a mockery of claims that the UK is in any way a partnership of equals.
European Commission president Jean-Claude Juncker said the deal was a "fair and balanced agreement for the EU and the UK and it is testament to our commitment to find solutions".
“The Brexit envisaged by Boris Johnson is one which sees a much looser relationship with the EU when it comes to issues like food standards, environmental protections and workers’ rights.“That is not the future that I or my government envisage for Scotland.
“And in the circumstances which now prevail it is clearer than ever that the best future for Scotland is one as an equal, independent European nation. That is a choice I am determined to ensure is given to the people of Scotland.”
The PM's spokesman confirmed plans to hold a meaningful vote on the deal this Saturday, which MPs approved in a vote on Thursday.
MPs from around the region have been reacting to the news. John Lamont said the deal is the best result for his constituents who "want to see Brexit sorted."
Copeland MP Trudy Harrison says she will be voting for the new deal on Saturday.
Mrs Harrison said: “Boris Johnson, against the odds, has managed to open the Withdrawal Agreement, get rid of the backstop and alter the political declaration – and has done it in 85 days.
“Workers, social and environmental rights will remain as the previous Agreement and we have a green light from the EU to develop our own world-beating Free Trade Agreement."
Liberal Democrat and Westmorland and Lonsdale MP Tim Farron is less pleased with the deal, tweeting: "Given that there will never be a unionist majority in the Stormont assembly, Mr Johnson’s proposed border in the Irish Sea will be permanent. It means the loss of Northern Ireland and then Scotland from the UK. This is short term benefit for BJ and long term harm for the country."
Scottish Secretary and MP for Dumfries Alister Jack said in a statement: “It is now time for Scottish MPs from all parties to vote for the Prime Minister’s deal and deliver the result of the referendum. It is time to put the national interest above political opportunism.''in response to the announcement of the deal agreement."
He continued, “Outside of the EU, Scotland and the whole of the UK will thrive. We have a bright future as a country. People now expect their elected representatives to do their duty to deliver that.”
The chance of the deal being passed in the UK relies on how many opposition MPs he can persuade to rebel against their party.