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The UK Government will reject a request from the Scottish Government for a second referendum on independence.
Scottish Secretary David Mundell said it would not enter into "discussions or negotiations" on a request for the legal power to hold a vote.
It comes after Prime Minister Theresa May said "now is not the time" for another ballot
Mr Mundell , MP Dumfriesshire Clydesdale & Tweeddale, said: "This argument isn't about mandates, it's about holding a referendum on established criteria which were set on in the Edinburgh Agreement.
"That established that a referendum must be legal, fair and decisive.
"The proposal brought forward is not fair, people will not be able to make an informed choice.
"Neither is there public or political support for such a referendum.
"Therefore we will not be entering into discussions or negotiations about a section 30 agreement and any request at this time will be declined."
Scotland's First Minister Nicola Sturgeon has announced she'll seek approval for a second independence referendum.
Lori Carnochan has been to gauge local reaction, and you can watch her video report here:
Borders MP Calum Kerr has said Prime Minister Theresa May could stop moves towards a second independence referendum if she reconsidered 'proposals for Scotland to stay within the Single Market'.
The SNP MP for Berwickshire, Roxburgh and Selkirk, said: "There is nothing inevitable about this process. If the Prime Minister is prepared to listen to the 64 per cent of Scots who voted to remain in Europe and seek compromise to accommodate these serious and pragmatic proposals, she could end this process tomorrow."
"Unlike in 2014, we know that change is coming either way. So the key question now is who we want to be in control of that change and what kind of country we want our children to grow up in," he added.
Latest ITV News reports
Mrs May accused First Minister Nicola Sturgeon and her party of being in a "muddle" about their plans to leave the UK.
The SNP's deputy leader has attacked the government's rejection of a second referendum on Scotland's future in the UK.