The World Owl Trust say their current agreement with the estate managers is being terminated and unless they can negotiate new arrangements, they'll have to leave by May 2015.
The charity claims the new terms are not financially viable, but the estate say negotiations are ongoing and in tough financial times it's having to review the way the owl centre is run.
The Muncaster estate says it's willing to negotiate but the Trust says it's looking for new premises. Honorary President Tony Warbuton said: "the owl trust is looking for somewhere new to be based, we do not feel we are wanted anymore at Muncaster."
Peter Frost-Pennington who runs Muncaster Castle says there will always be a home for owls on their site.
But he says they have been forced to review the way the owl centre is run by the World Owl Trust due to a weak economy.
"We are committed to the conservation work that the centre does, as well as the wider conservation work we undertake at Muncaster.
"The last few years have been difficult for Muncaster, like many other tourist attractions, due to the weak economy and we need to make sure that every part of the attraction is operating effectively.
"As part of this we are reviewing the way the owl centre is run. That is what we have said we would like to discuss with the World Owl Trust, which currently runs the centre, and other organisations."
He added their aim is to continue having an owl centre at Muncaster that works in the best interest of the owls and visitors.
One of Cumbria's leading tourist attractions could be set for a new home.
The World Owl Trust claims potential changes to its lease could force it out of Muncaster Castle.
The castle says it wants to keep an owl centre, but needs to look at efficiency.
The World Owl Trust, which is based at Muncaster Castle in Cumbria, is urging people to grow their grass to help the Barn Owl. The population has plummeted in Britain due to a loss of habitat and the Trust say 'welly high grass' will help boost numbers. Hannah McNulty went to find out why.
World Owl Trust conservation officer, Millie Clarke, explains what the 'Welly high grass' campaign is all about:
An event aimed at raising awareness about owls and how important they are is being held by the World Owl Trust at Muncaster Castle.
They will be offering information on how to help owls living around the region and will be asking hoteliers to allow their grass to grow long, which will encourage young animals into their gardens- providing a vital source of food for owls.
"Our Conservation Day is a great opportunity for people to come and see the kinds of things that they can do to make a real difference in helping see owls thrive in their area."
The owl trust centre is home to more than 200 owls from 50 different species and sub-species.
Fifty-five photos of rural life are going on display throughout the Eskdale Valley in the western Lake District. The pictures will be shown beside waterfalls, along footpaths, at farms and at several stations on the Ravenglass and Eskdale railway on the 8th and 9th September.