Six more people in the Calendar region who tested positive for coronavirus have died.
It comes as the number of people to have died from coronavirus in the UK jumped by more than 100 to 578 - in the biggest daily increase to date.
Medics say almost 600 people in Yorkshire and Lincolnshire have been diagnosed with the virus since the outbreak began.
But they are likely to be just a fraction of the true number because tests are only being carried out on hospital patients.
Sheffield has now recorded 240 cases - four people have died in hospital there in recent days.
The latest figures reveal two more patients have died - in York and Chesterfield.
The number of patients diagnosed with coronavirus in the region has risen by 135 from 462 yesterday - to 597 today.
The Department of Health says the size of the increase in part reflects a change in the timeframe it reports the figures.
As of 9am on 26 March, a total of 104,866 people have been tested, of which 93,208 were confirmed negative and 11,658 were confirmed positive. 578 patients have died across the UK.
Sheffield 240 (174)
Leeds 67 (53)
North Yorkshire 50 (45)
Lincolnshire 32 (31)
Rotherham 30 (20)
Barnsley 27 (19)
Kirklees 24 (20)
Wakefield 24 (19)
Bradford 23 (16)
East Yorkshire 21 (19)
Doncaster 21 (15)
York 14 (14)
Calderdale 9 (7)
North Lincolnshire 6 (5)
North East Lincolnshire 5 (3)
Hull 4 (2)
The first figure is the cumulative confirmed cases by local authority up to today, provided by Public Health England. The figure in brackets is the total up to yesterday.