Atomic Kitten were joined by a brass band from Leeds to perform their revamped version of Whole Again ahead of England's semi-final against Denmark.
The girl group - Liz McClarnon, Jenny Frost and Natasha Hamilton - announced on Tuesday that they were re-releasing their 2001 single with a special twist for Euro 2020.
Hyde Park Brass band gave a public performance of the track, called Southgate You're The One (Football's Coming Home Again) in Leicester Square, central London, on Wednesday morning.
The song has become a crowd anthem at games, with fans changing the lyrics to: "Southgate you're the one, you still turn me on, football's coming home again."
The famous girl group said:
"It's been a whirlwind couple of days, but we are super excited and grateful to be able to contribute to the awesome energy and patriotism that is filling the England streets with this version of Whole Again.
"Totally inspired by the England football fans in support of Gareth Southgate and the super talented England football team, we will be singing loud and proud 'Football's coming home'."
Atomic Kitten formed in 1998 and released hits including The Tide Is High and Eternal Flame, and have featured a shifting line-up including singers Kerry Katona and Heidi Range.