Insp John Lawson, of Humberside Police, confirmed the collision happened on the Aire and Calder bridge, over the canal. He said the two drivers were pronounced dead at the scene along with a front seat passenger from one of the vehicles.
"Police are requesting that any witnesses to the collision or that had travelled over the bridge at the time of the collision to contact Humberside Police.
The officer said the force will not be releasing any further information until tomorrow.
The family of Derek and Ethan Sarkar, who were killed in a crash near Goole, say their loss is beyond belief.
A nine year old girl and her mother are still in hospital after a crash which killed her father and brother.
Police have released details of the three people killed and the two seriously injured in a head on collision in East Yorkshire.