Chester Zoo staff say they are "devastated" some animals died in Saturday's fire.
The blaze broke out in the roof of the Monsoon Forest area, leading to an evacuation of visitors.
Today the zoo confirmed insects, frogs, fish and small birds had died in the fire.
At this stage it's thought the fire started accidentally
An appeal for donations had already raised nearly 75 thousand pounds.
This report from Ralph Blunsom
Fire investigators say the fire at Chester Zoo at the weekend was caused by an electrical fault.
Chester Zoo has thanked all those who have donated after a fire killed a number of animals - vowing to continue its conservation mission.