London City Airport will suspend its operations until the end of April, during a collapse in demand caused by the coronavirus.
The airport said it will close its runway to commercial and private flights from Wednesday night.
London City is the UK's 12th busiest airport, handling 5.1 million passengers last year.
Its location close to the capital's financial district makes it particularly popular with business travellers.
The airport is used for British Airways' business-class only flights to and from New York.