Work to dismantle the 15-mile contraflow system on the M20 in Kent, put in place as part of Operation Brock, will begin on Monday.
The system was implemented in March 2019 to prevent severe delays on the motorway, which is the key freight route to and from Dover, in the event of a no-deal Brexit.
But it was announced that the steel barrier, between Junction 8 for Maidstone and Junction 9 at Ashford, would be removed because the risk of a no-deal Brexit has lowered.
The decision was made by Highways England, the Kent Resilience Forum and the Department for Transport.
It cost £35 million to put in place, which includes the design, construction, testing, activation and deactivation of the system.
Meanwhile, the cost of commissioning extra ferries and preparing the Manston Airport site as a potential lorry park means the total spend on no-deal Brexit planning in Kent has reached £123 million.
The M20 will be fully closed in both directions between Junction 7 and 9 from 8pm on Monday 13th January until 6am on Tuesday 14th January.
Then from 8pm on Tuesday 14th January, the London-bound carriageway of the motorway will be closed between Junction 7 and 9 until 6am every night for a further 15 nights.
For full details of the closures click here.
ITV News Meridian has approached the Government for comment about the cost of Brexit preparations in Kent, but no-one has been available for comment.