Five members of staff at a nursery on the Isle of Wight considered quitting the day after an Ofsted inspection was carried out.
The individuals at the nursery in Newport said they were close to handing in their notices the following morning, describing the latest visit as "very different" to previous ones due to an increased level of scrutiny.
Emma Corina, Director of Housing and Development, YMCA Fairthorne Group has been in her role for 17 years.
Over this time she has experienced several Ofsted visits but said on the last two occasions, staff said the inspectors had "come with the mindset of finding something wrong".
The nursery is now contesting the result of the Ofsted inspection, which Emma said is "much much lower than we were expecting".
Emma said: "It's really really stressful. It's very subjective depending on which inspector comes and how they conduct themselves and we've had experiences at both ends of the scale. These two most recent ones have felt very punitive. The staff have been extremely upset and they've found the experience very distressing.
"The latest one on the Isle of Wight we had five resignations the next morning. We had staff in tears...as a result of this. We've had to spend that period since the inspection trying to help them recover but they're still very distressed about the experience.
"What we would really like Ofsted to do is to commission independent research into two factors. One, does the evidence say their inspections have improved quality. Two, does the methodology of their inspection framework, which feels punitive, does that actually affect change or does it make people feel fearful?"
Emma Corina, Director of Housing and Development, YMCA Fairthorne Group
At the National Association of Head Teachers (NAHT) annual conference on Friday (April 28), the union chief is due to urge schools to remove Ofsted banners from their railings to put the inspectorate "back in its box."
Simon Kidwell, president elect of the NAHT, will say that the current inspection framework "isn't fit for purpose"and is "doing more harm than good".
In a speech to hundreds of school leaders at the union's annual conference, Mr Kidwell will say the current model "causes unacceptable collateral damage" to mental and physical health.
Pressure has been mounting on the schools watchdog in England to introduce urgent reforms to the inspection process.
It comes after the death of Ruth Perry, headteacher at Caversham Primary School in Reading, Berkshire, who took her own life in January while awaiting an Ofsted report which downgraded her school from the highest rating to the lowest possible.
Speaking at the Telford International Centre on Friday, Mr Kidwell will say that an Ofsted inspection in his school last week "almost broke two valuable and brilliant members of staff".
Mr Kidwell, who is principal of Hartford Manor Primary School and Nursery in Cheshire, will say to school leaders: "I know we will all have our own stories to tell about how the punitive accountability system has affected us personally.
"My own physical health has been impacted by the job - 16 months ago I was rushed into hospital and for three days was signed off work because of physical complications that doctors thought was directly caused by the stresses and pressures of work.
"Ruth (Perry)'s death has sadly made me question if I have enough in the tank to lead my school through another Ofsted cycle."
Addressing 400 school leader delegates at the union's annual conference, Mr Kidwell will say: "The reality for many is that the current approach to inspection compounds inequality between schools.
"The current model is a workload creation vehicle for subject leaders, and it causes unacceptable collateral damage to school leaders' mental and physical health."
He will add: "When we return to school on Tuesday, let's take some collective actions to put Ofsted back in its box.
"Let's remove any Ofsted banners from our railings, erase Ofsted logos from our school stationery, delete Ofsted quotes from our websites, and when Ofsted reports are published tell our communities that Ofsted is a snapshot of school performance judged against a framework that urgently needs a serious reform."
Last month, some schools began removing logos and references to Ofsted ratings from their websites as a mark of solidarity with Ms Perry.
Ofsted chief Amanda Spielman has said she has no "reason to doubt" the inspection before the death of Ms Perry.
Speaking on the BBC's Laura Kuenssberg On Sunday programme, Ms Spielman acknowledged that a culture of fear exists around inspections but she said the majority of schools have a "positive and affirming experience".
An inspection report, published on Ofsted's website in March, found Ms Perry's school to be "good" in every category apart from leadership and management, where it was judged to be "inadequate".
Professor Julia Waters, Ms Perry's sister - who previously said the grading was "sensationalist" and "deeply harmful", is due to speak at the NAHT union's annual conference on Saturday.
Ms Spielman has said Ofsted's one-word assessments, which have been criticised for being too simplistic, are easier for parents to understand.
She said on Sunday: "It's not for us to say we're going to fundamentally change the grading system, that would have to be a bigger government decision."
Last week, Education Secretary Gillian Keegan said: "Parents rightly want to know how their child's school is doing and I fully support our approach to providing a clear one-word rating to inform their decisions."
The NAHT will ballot its members in England over strike action after members rejected the Government's pay offer.
Delegates at the conference in Telford will vote on whether to endorse the decision by the NAHT's executive committee to re-ballot members.
More than three-quarters (78%) of NAHT members who voted said they would be prepared to vote for action up to and including strikes.
Mr Kidwell, who will become the NAHT's official president in September, will say: "A demonstrable signal that we won't stand by and let the long-term erosion in terms and conditions and the invisible hand of toxic accountability and underfunding continue to suffocate our profession."
In a statement, an Ofsted spokesperson said: "Our inspections are first and foremost for children and their parents – looking in depth at the quality of education, behaviour, and how well and safely schools are run. We always want inspections to be constructive and collaborative and in the vast majority of cases school leaders agree that they are.
"We have no issue with schools removing Ofsted logos and banners, especially if it helps diffuse some of the anxiety around inspection. Many schools understandably want to celebrate their success, but they are under no obligation to display their Ofsted grade and we certainly don’t ask them to."