Cyclists in Windsor and Eton who ignore no cycling signs will soon be issued with £50 fines.
Thames Valley Police Neighbourhood Team says it has become aware of a number of people who have been riding bicycles in no cycling zones and there have also been issues with people cycling the wrong way down one way streets.
The tougher rules have been introduced after a four year old boy was knocked down and seriously injured by a cyclist in a pedestrian area.
A man was jailed for 27 weeks for causing bodily harm by wilful misconduct when driving.
Police have issued a video of the incident.