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Only membership of NATO would enable Ukraine to defend itself from external aggression, Ukrainian Prime Minister Arseny Yatseniuk said.
Speaking at a conference in Kiev attended by Ukrainian and European lawmakers and business leaders on Saturday, Yatseniuk made clear he did not view the ceasefire as the start of a sustainable peace process because of Putin's ambitions.
Ukraine is "still in a state of war" with neighbouring Russia despite a cease-fire between Kiev's forces and Moscow-backed rebels in the east, the country's prime minister said as a second convoy of Russian trucks rolled into Ukraine.
Speaking at a conference with politicians and business leaders in Kiev, Arseniy Yatsenyuk said Russian President Vladimir Putin's goal is "to take the entire Ukraine".
Donetsk city council said there had been fighting near the airport overnight. Two shells hit homes in the area but there were no reports of casualities.
Heavy artillery has been reported across northern Donetsk in eastern Ukraine, and a large plume of black smoke has been seen by eyewitnesses, Reuters reports.
Donetsk airport remains in the hands of Ukrainian government forces, while the city is controlled by pro-Russian separatists.
Earlier, the Ukrainian military said the rebels had fired on their forces overnight in the area of the airport.
Russian President Vladimir Putin wants to destroy Ukraine as an independent country and restore the Soviet Union, the country's prime minister claimed today.
Arseny Yatseniuk also praised a new wave of economic sanctions imposed on Russia by the European Union and the United States and said they posed a major threat to the Russian economy.
"We are still in a stage of war and the key aggressor is the Russian Federation ... Putin wants another frozen conflict (in eastern Ukraine)," he said at an conference in Kiev attended by European and Ukrainian politicians and business leaders.
"His goal is to take the entire Ukraine ... Russia is a threat to the global order and to the security of the entire Europe," Yatseniuk added.